Regeneration of plantlets from <i>in vitro </i>raised leaf explants of <i>Cleisostoma racimeferum </i>Lindl

Abstract

Protocorm like bodies (PLBs), callus and shoot buds developed in culture from <i>in vitro </i>raised foliar explants of <i>Cleisostoma racimeferum. </i>Among the different basal media, better result was obtained on MS medium containing sucrose (3%) and BA (2 μ<i>M</i>) with ~80% frequency after 40 days of culture. Young leaves (15 week old) produced better PLBs. Whole leaf placed vertically upside-up orientation can regenerate PLBs and shoot buds (80%). PLBs and shoot buds formed on entire surface of the leaves. Cultures on BA and NAA (2 and 2 μ<i>M</i> respectively in combination) stimulated callus mediated regeneration (68%). The rooted plantlets regenerated within 8-10 week from PLBs and shoot buds on MS medium containing IAA and kinetin (2 μ<i>M</i> each in combination). BA containing medium triggered multiple shoot bud formation, while NAA alone or in combination with other growth regulators was inhibitory. Incorporation of activated charcoal (0.01 %) in the medium stimulated formation of repetitive PLBs and multiple shoot buds. Rooted plants were ready for harvest after 20-22 week of initiation of culture. About 65% of the potted plants survived after 3 months in the poly house.