Comparative diagnostic potentiality of ELISA and Dot -ELISA in prepatent diagnosis of experimental <i>Fasciola gigantica</i> infection in cattle

Abstract

A glycoprotein (27 kDa) was isolated from crude somatic antigen of <i>Fasciola gigantica</i> by two steps affinity chromatography and was used in early detection of experimental fasciolosis in cattle by indirect ELISA and in dot-ELISA formats. Although, anti-27 kDa antibodies could be detected after 3 weeks post infection (WPI) by dot - ELISA which was one week later than indirect ELISA. The test, dot-ELISA, was more convenient in field application. By the test (dot-ELISA) the infection could be equally detected in animals infected with 100, 200 and 300 metacercariae of <i>F. gigantica</i> with high sensitivity. Further, the antigen (27 kDa) was not found to react with goat sera infected with <i>Paramphistomum epiclitum</i>, which are giving strong reaction to homologous immature and mature fluke antigens of <i>P</i>. <i>epiclitum</i>.