<i>In vitro </i>multiple shoot regeneration and plant production in <i>Alysicarpus rugosus</i> DC. var. <i>heyneanus </i>Baker

Abstract

A protocol for <i>in vitro </i>multiple shoot regeneration and plant production through seedling (shoot tip) culture was established for <i>Alysicarpus rngosus </i>DC. var. <i>heyneanus </i>Baker. Maximum number of adventitious shoots (14.4) per shoot tip explant were initiated after two subcultures on MS solid medium supplemented with IAA (2.85 μ<i>M</i>) plus BAP (2.22 μ<i>M</i>) after 4 weeks. Shoot elongation (3.0-3.5 cm) was achieved on MS medium without any hormones. Stunted shoots elongated on half MS medium without growth hormones. Rooting occurred in MS medium containing IAA (1.14-2.85μ<i>M</i>) alone or in combination with IBA (0.89-2.46μ<i>M</i>) and or NAA (1.07-2.69μ<i>M</i>). Maximum rooting was established in MS medium supplemented with IAA (2.85μ<i>M</i>). The plants were acclimatized successfully with 55% survival in pot containing cocoa peat and sand (1:1). After a month, hardened plants were transferred to pots with manure, garden soil and sand (1:2:1) for further growth and finally planted in field.