Antioxidant and nitric oxide synthase activation properties of <i>Ganoderma</i> <i>applanatum</i>

Abstract

<i>In vitro </i>evaluation of antioxidant activities of <i>Ganoderma</i> <i>applanatum</i> showed significant inhibition of lipid peroxidation,<i> </i>and potent hydroxyl radical scavenging activity when compared<i> </i>with standard drug catechin. IC₅₀ values of crude, boiled and<i> </i>ethanolic extracts of <i>G</i>. <i>applanatum </i>were 604.8, 624 and 267<i> μ</i>g/ml,<i> </i>respectively in case of hydroxyl radical scavenging<i> </i>activity, and 441, 520.5 and 166.16 <i>μ</i>g/ml,<i> </i>respectively in case of<i> </i>lipid peroxidation. Furthermore, crude, boiled and ethanoiic<i> </i>extracts also increased significantly nitric oxide production<i> </i>(156.67, 121.88 and 742 pmole/mg dry wt/hr, respectively) over

the control. The results of present investigation revealed that <i>G</i>. <i>applanatum </i>have potential therapeutic use.