Effectivity of crude versus purified mycobacterial secretory proteins as immunogen for optimum antibody production

Abstract

Monospecific antibodies have been successfully utilized in antigen detection, which is better indicator of active infection. <i>Mycobacterium tuberculosis </i>excretory secretory (<i>M tb </i>ES)

antigens such as ES 31 , ES 41 and ES 43 (31 kDa, 41 kDa and 43 kDa protein, respectively) have been shown to be present in <i>Mycobacterium tuberculosis </i>H₃₇Ra culture filtrate and are of diagnostic interest. To study the immunogenic potential of crude versus purified antigen, goat was immunized with <i>M tb </i>detergent soluble sonicate (DSS) antigen as well as purified antigen fraction (ESAS 7) containing ES 31 antigen. Both anti-DSS IgG antibody and anti ESAS 7 IgG antibody were found to be reactive with ES 31 antigen upto 1 ng concentration of antibody by ELISA. Crude DSS antigen was found to be quite effective in producing high

titre antibodies and showed further high reactivity with other ES antigens (ES 41 and ES 43) of diagnostic interest.