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http://nopr.niscpr.res.in/handle/123456789/23330| Title: | Standardization of the method for estimation of ethambutol in pharmaceutical preparations and biological fluid |
| Authors: | Gurumurthy, Prema Gayathri, T N Bhagavathy, S Venkatesan, P |
| Keywords: | Ethambutol;Amberlite CG-50;Rifampicin;Isoniazid;Pyrazinamide |
| Issue Date: | Jan-2004 |
| Publisher: | NISCAIR-CSIR, India |
| Abstract: | A simple column chromatographic method for determination of ethambutol (EMB) in pharmaceutical preparations containing EMB in combination with other anti-TB drugs is presented. The method involved extraction of EMB into an organic solvent, followed by basification and column chromatographic separation on Amberlite CG 50 (100-200 mesh) and elution with suitable eluants and estimation at a wavelength of 270 nm. The assay was linear from 25 to 400μg/ml. The relative standard deviations of intra and inter day assays were lower than 5%. Ethambutol was recovered from human urine quantitatively and stable for a period of atleast one week in urine stored at-20°C. |
| Page(s): | 68-73 |
| ISSN: | 0975-1009 (Online); 0019-5189 (Print) |
| Appears in Collections: | IJEB Vol.42(01) [January 2004] |
Files in This Item:
| File | Description | Size | Format | |
|---|---|---|---|---|
| IJEB 42(1) 68-73.pdf | 1.37 MB | Adobe PDF | View/Open |
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