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http://nopr.niscpr.res.in/handle/123456789/23429| Title: | In vitro propagation of emetic nut Randia dumetorum (Lamb.) |
| Authors: | Begum, Ferdousi Didarul Islam, Kazi Mohammed Paul, Rathindra Nath Mehedi, Masfique Rani, Shyamole |
| Keywords: | Endangered species;In vitro propagation;Randia dumetorum;Shoot tip;Tribes |
| Issue Date: | Dec-2003 |
| Publisher: | NISCAIR-CSIR, India |
| Abstract: | An efficient protocol for in vitro shoot multiplication of Randia dumetorum (Emetic nut) has been developed. The seeds of R. dumetorum were germinated in vitro in MS medium in 5 weeks. Subsequent propagation using shoot tip as an explant was carried out in MS medium along with different concentrations and combinations of BAP (0.5-2.0) and NAA (0.0-2.0). Maximum shoot multiplication was obtained (12.7 shoots per shoot tip) in MS medium containing 1mg/L BAP and 1mg/L NAA. Micropropagated shoots were rooted in 1/2MS medium supplemented with 1 mg/l IBA. This is the first report of in vitro plant propagation of R. dumetorum. In vitro grown plantlets showed a survival rate of 70% after 2 months of tranplantation to natural environment. |
| Page(s): | 1479-1481 |
| ISSN: | 0975-1009 (Online); 0019-5189 (Print) |
| Appears in Collections: | IJEB Vol.41(12) [December 2003] |
Files in This Item:
| File | Description | Size | Format | |
|---|---|---|---|---|
| IJEB 41(12) 1479-1481.pdf | 689.58 kB | Adobe PDF | View/Open |
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