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| DC Field | Value | Language |
|---|---|---|
| dc.contributor.author | Syamala, D | - |
| dc.contributor.author | Devi, Prathibha | - |
| dc.date.accessioned | 2013-11-12T10:52:42Z | - |
| dc.date.available | 2013-11-12T10:52:42Z | - |
| dc.date.issued | 2003-12 | - |
| dc.identifier.issn | 0975-1009 (Online); 0019-5189 (Print) | - |
| dc.identifier.uri | http://hdl.handle.net/123456789/23431 | - |
| dc.description | 1482-1486 | en_US |
| dc.description.abstract | Novel protocols for production of multiple shoot-tip clumps and somatic embryos of Sorghum bicolor (L.) Moench were developed with long-term goal of crop improvement through genetic transformation. Multiple shoot-tip clumps were developed in vitro from shoot-tip explant of one-week old seedling, cultured on MS medium containing only BA (0.5, 1 or 2 mg/l) or both BA (1 or 2 mg/I) and 2,4-D (0.5 mg/l) with bi-weekly subculture. Somatic embryos were directly produced on the enlarged dome shaped growing structures that developed from the shoot-tips of one-week old seedling explants (without any callus formation) when cultured on MS medium supplemented with both 2,4-D (0.5 mg/l) and BA (0.5 mg/I). However, the supplementation of MS medium with only 2, 4-D (0.5 mg/l) induced compact callus without any plantlet regeneration. Each multiple shoot-clump was capable of regenerating more than 80 shoots via an intensive differentiation of both axillary and adventitious shoot buds, the somatic embryos were capable of 90% germination, plant conversion and regeneration. The regenerated shoots could be efficiently rooted on MS medium containing indole-3-butyric acid (IBA 1 mg/l). The plants were successfully transplanted to glasshouse and grown to maturity with a survival rate of 98%. Morphogenetic response of the explants was found to be genotypically independent. | en_US |
| dc.language.iso | en_US | en_US |
| dc.publisher | NISCAIR-CSIR, India | en_US |
| dc.rights |  CC Attribution-Noncommercial-No Derivative Works 2.5 India | en_US |
| dc.source | IJEB Vol.41(12) [December 2003] | en_US |
| dc.subject | Regeneration | en_US |
| dc.subject | Shoot-tip explants | en_US |
| dc.subject | Sorghum | en_US |
| dc.title | Efficient regeneration of sorghum, Sorghum bicolor (L.) Moench, from shoot-tip explant | en_US |
| dc.type | Article | en_US |
| Appears in Collections: | IJEB Vol.41(12) [December 2003] | |
Files in This Item:
| File | Description | Size | Format | |
|---|---|---|---|---|
| IJEB 41(12) 1482-1486.pdf | 1.23 MB | Adobe PDF | View/Open |
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