<i style="mso-bidi-font-style: normal">In vitro</i> propagation of a rare medicinal fern of Western Ghats <i style="mso-bidi-font-style:normal">– Diplazium esculentum</i> (Reytz.)

Abstract

Present study aimed for <i style="mso-bidi-font-style: normal">in vitro</i> culture of circinate part of young leaves of <i style="mso-bidi-font-style:normal">D. esculentum </i>which is amongst the leafy<i style="mso-bidi-font-style:normal"> </i>vegetables consumed as vegetable by Paniya and Chetti tribes of Western Ghats. The circinate part of young leaves (crosiers), excised before the beginning of foliar expansion, was inoculated on half strength Murashige and Skoog (MS) medium supplemented with auxins indole-3-butyric acid (IBA) or α-napthalene acetic acid (NAA) or 2,4-Dichlorophenoxyacetic acid (2,4-D) and cytokinin 6- benzylaminopurine (BA) in a range 0.5 to 2.5 mg L^-1. Combinations of different concentrations of 2,4 D + BA, IBA + BA as well as of NAA+ BA were also tested in half strength MS medium with 3% sucrose and with <i style="mso-bidi-font-style: normal">p</i>H 5.8. The best morphogenic response was obtained with half strength MS medium supplemented with 2,4-D 0.5 mg L^-1 and BA 2.5 mg L^-1, 3% sucrose, at <i style="mso-bidi-font-style:normal">p</i>H 5.8. For rooting of the microshoots, half strength MS medium supplemented with 2,4-D ( 2 and 1 mg L^-1 ) exhibited best results. Present study reports the successful <i style="mso-bidi-font-style:normal">in vitro</i> culturing of <i style="mso-bidi-font-style:normal">D. esculentum</i>.