Biosynthesis of colloidal gold nanoparticles by <i style="mso-bidi-font-style:normal">Streptomyces</i> sp. NK52 and its anti-lipid peroxidation activity

Abstract

Gold nanoparticles (Au-NPs) were synthesized from chloroauric acid using cell free supernatant of <i style="mso-bidi-font-style: normal">Streptomyces</i> sp. NK52 grown in nutrient broth. These nanoparticles were synthesized by varying pH and temperature of the reaction mixture and chloroauric acid concentration. The nanoparticles were characterized by spectrometry, X-ray diffraction, Scanning electron microscopy and energy dispersive spectrometry. Au-NP ranged from 10-100 nm in size and exhibited a polydispersive nature with various shapes like rods, hexagons, triangles, spheres. The diffraction peaks at 2θ = 38.1◦ and 44.5◦ could be assigned to the (1 1 1) and (2 0 0) planes of a faced centre cubic (fcc) lattice of gold. Au-NP showed 47% inhibition of lipid peroxidation <i>in</i> <i>vitro</i>. To the best of our knowledge, this is the first report on the rapid biosynthesis of Au-NP using cell free supernatant of <i style="mso-bidi-font-style:normal">Streptomyces</i> sp. and its evaluation for anti-lipid peroxidation.