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http://nopr.niscpr.res.in/handle/123456789/23625| Title: | In vivo and in vitro influence of selenium on DNA/RNA synthesis in spleen and lymphocytes in culture-Possible mediation of changes in GSH/GSSG ratio |
| Authors: | Bansal, M P Sood, Shaloo |
| Issue Date: | Jan-2001 |
| Publisher: | NISCAIR-CSIR, India |
| Abstract: | Effect of superanutritional levels of selenium (Se) as sodium selenite (0.5 and 1.5 ppm) given orally to Balb/c mice for one and two weeks was observed on the rate of DNA/RNA synthesis, levels of reduced as well as oxidized glutathione (GSH and GSSG) and glutathione peroxidase (GSH-Px) /glutathione-S-transferase (GSH-S-transferase) activities in spleen. Similar effect of three different concentrations of Se (10-7, 10-5 and 10-3 M) in culture media was also observed on the rate of DNA/RNA synthesis in proliferating lymphocytes taken from mice spleen. The results of the present study indicated that with increasing concentration and duration of Se treatment in vivo and in vitro, a marked inhibition of the rate of DNA/RNA synthesis was observed. Levels of total glutathione and GSSG in spleen were elevated significantly only after two weeks in 1.5 ppm treatments. Glutathione peroxidase activities in spleen decreased (p<0.05) in 1.5 ppm group at one week and in 0.5 ppm group at two week treatment. At higher Se treatment, the activity recovered towards control. However, GSH-S-transferase in spleen remained unchanged at all treatment intervals. The results indicated that changes in glutathione system by increasing Se concentration might account for inhibition of rate of DNA/RNA synthesis. |
| Page(s): | 25-28 |
| ISSN: | 0975-1009 (Online); 0019-5189 (Print) |
| Appears in Collections: | IJEB Vol.39(01) [January 2001] |
Files in This Item:
| File | Description | Size | Format | |
|---|---|---|---|---|
| IJEB 39(1) 25-28.pdf | 1.01 MB | Adobe PDF | View/Open |
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