Protection against rotavirus diarrhoea in mice by trypsin inhibitor

Abstract

<span style="font-size: 13.5pt;mso-bidi-font-size:8.5pt;font-family:" times="" new="" roman","serif";="" color:black"="">To investigate the role of soyabean trypsin inhibitor (TI) during rotavirus (RV) diarrhoea, changes in enzyme activities of six relevant mucosal enzymes (lactase, sucrase, maltase, trehalase, glucoamylase and alkaline phosphatase) were assayed following inoculation of suckling mice with EB rotavirus (serotype 3) along with the TI and compared with the agematched healthy control mice. The animals were divided into three groups i.e. group 1 (controls), group 2 (RV inoculated)

<span style="font-size:13.5pt;mso-bidi-font-size:8.5pt;line-height:115%; font-family:" times="" new="" roman","serif";mso-fareast-font-family:"times="" roman";="" color:black;mso-ansi-language:en-us;mso-fareast-language:en-us;mso-bidi-language:="" ar-sa"="">and group 3 (RV <span style="font-size:14.5pt;mso-bidi-font-size: 9.5pt;line-height:115%;font-family:" arial","sans-serif";mso-fareast-font-family:="" "times="" new="" roman";color:black;mso-ansi-language:en-us;mso-fareast-language:="" en-us;mso-bidi-language:ar-sa"="">+ <span style="font-size:13.5pt; mso-bidi-font-size:8.5pt;line-height:115%;font-family:" times="" new="" roman","serif";="" mso-fareast-font-family:"times="" roman";color:black;mso-ansi-language:en-us;="" mso-fareast-language:en-us;mso-bidi-language:ar-sa"="">TI inoculated and sacrificed under light anaesthesia on 0,1,3,5, 7 and 10 day post inoculation (dpi). Then intestines were excised and divided into two parts jejunum and ileum). They were separately homogenized in 0.9% cold normal saline and activities of mucosal enzyme were measured. Alkaline phosphatase and disaccharidases were found to be decreased significantly in RV inoculated animals in both the anatomical portions of small intestine of mice. These enzyme levels were restored with the administration of TI i.e. in group 3 and became comparable to the controls in both intestinal portions. These studies suggest that activity of intestinal enzymes which are important in digestive absorptive functions of small intestine were restored with the addition of TI when given to infant mice showing its protective efficacy during rotavirus infection.</span></span></span></span>