Please use this identifier to cite or link to this item: http://nopr.niscpr.res.in/handle/123456789/23809
Title: Occurrence of chromium resistant thermotolerant coliforms in tannery effluent
Authors: Verma, Tuhina
Ramteke, Pramod W
Garg, Satyendra K
Keywords: Antibiotic resistance;Conjugation;Escherichia coli;Heavy metals;Plasmid;Tannery effluent;Thermotolerant E. coli
Issue Date: Nov-2004
Publisher: NISCAIR-CSIR, India
Abstract: Twenty six thermotolerant strains resistant to high levels of chromium (50-250 μg/ml) were isolated from treated tannery effluent. They were also found resistant to multiple heavy metals and antibiotics. Majority of them were resistant to copper and bacitracin. Nine strains representing different resistance patterns were selected for plasmid profile and conjugation studies. Agarose gel electrophoresis results revealed that 6 strains harboured a single plasmid, whereas 3 strains exhibited 2 plasmid bands. Among antimicrobials, co-trimazole and bacitracin and among metals, Cu2+, Cd2+, Zn2+ and Ni2+ resistance were transferred most frequently at variable rates. However, chromium resistance was transferred in 6 strains with a frequency ranging 19-49×10-2. Resistance to Co2+ and Hg2+ did not transfer under environmental conditions. Among the nine strains, three were found predominantly uropathogenic Escherichia coli (UPEC) serotype 04, whereas two strains were untypable. In addition, 4 transconjugants also showed a positive result after serotyping.
Page(s): 1112-1116
ISSN: 0975-1009 (Online); 0019-5189 (Print)
Appears in Collections:IJEB Vol.42(11) [November 2004]

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