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http://nopr.niscpr.res.in/handle/123456789/23834| Title: | Immunological characteristics of PEP carboxylase from leaves of C3, C4- and C3C4 intermediate species of Alternanthera - Comparison with selected C3 and C4- plants |
| Authors: | Gayathri, J Parvathi, K Chinthapalli, Bhaskarrao Westhoff, Peter Raghavendra, A S |
| Issue Date: | Jul-2001 |
| Publisher: | NISCAIR-CSIR, India |
| Abstract: | Immunological cross-reactivity of phosphoenolpyruvate carboxylase (PEPC) in leaf ex tracts of C3-, C4- and C3-C4 intermediate species of Alternanthera (along with a few other C3- and C4- plants) was studied using anti-PEPC antibodies raised against PEPC of Amaranthus hypochondriacus (belonging to the same family as that of Altemanthera, namely Amaranthaceae) . Antibodies were also raised in rabbits against the purified PEPC from Zea mays (C4 - monocot - Poaceae) as well as Alternanthera pungens (C4 - dicot - Amaranthaceae). Monospecificity of PEPC-antiserum was confirmed by immunoprecipitation. Amount of PEPC protein in leaf extracts of A. hypochondriacus could be quantified by single radial immunodiffusion. Cros- reactivit y of PEPC in leaf extracts from selected C3-, C4-, and C3C4 intermediate species (including those of Alternanthera) was examined using Ouchterlony double diffusion and Western blots. Anti-PEPC anti serum raised against A. hypochondriacus enzyme showed high cross-reactivity with PEPC in leaf extracts of A. hvpochondriacus or Amaranthus viridis or Alternanthera pungens (all C4 dicots), but limited cross-reactivity with that of Zea mays, Sorghum or Pennisetum (all C4 monocots). Interestingly, PEPC in leaf extracts of AIternathera tenella. A. ficoides,. Parthenium hysterophorus (C3-C4 intermediates) exhibited stronger cross-reactivity (with anti-serum raised against PEPC from Amaranthus hypochondriacus) than that of Pisum sativum, Commelina benghalensis, Alternanthera sessilis (C3 plants). Further studies on cross-reactivities of PEPC in leaf ex tracts of these plants with anti-PEPC antisera raised against PEPC from leaves of Zea mays or Altemanthera pungens confirmed two points - (i) PEPC of C3-C4 intermediate is distinct from C3 species and intermediate between those of C3- and C4-species; and (ii) PEPC of C4-dicots was closer to that of C3species or C3C4 intermediates (dicots) than to that of C4-monocots. |
| Page(s): | 643-649 |
| ISSN: | 0975-1009 (Online); 0019-5189 (Print) |
| Appears in Collections: | IJEB Vol.39(07) [July 2001] |
Files in This Item:
| File | Description | Size | Format | |
|---|---|---|---|---|
| IJEB 39(7) 643-649.pdf | 1.52 MB | Adobe PDF | View/Open |
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