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http://nopr.niscpr.res.in/handle/123456789/23857| Title: | Isolation, purification and partial chemical characterization of a lethal factor from common Indian toad (Bulo melanostictus, Schneider) skin extract |
| Authors: | Das, Manika Dasgupta, S C Gomes, A |
| Issue Date: | Aug-2001 |
| Publisher: | NISCAIR-CSIR, India |
| Abstract: | Indian toad (Bufo melanosticus, Schneider) skin extract (TSE) is pharmacologically potent and probably contains several bioactive compounds [Das et. al., Indian J Pharmacal, 28 (1996) 72). A lethal factor was isolated and purified by neutral alumina column chromatography followed by HPLC. Spectroscopic (UV, IR, FAB-MASS) study indicated that the lethal factor (TSE-LF) was a 254 Da long chain compound with carbonyl, hydroxyl and ester as functional groups. LD50 of TSE-LF was found to be 3.5 mg/kg (iv). Biological study showed that TSE-LF possesses hypotensive, cardiotoxic, neurotoxic activity and produced death by apnoea in experimental animal. Cyproheptadine antagonised TSE-LF induced contraction of isolated smooth muscle indicating involvement of histamine/serotonin receptors. TSE-LF induced neurotoxic action on chick biventer cervices was mediated through Ca2+ ion. |
| Page(s): | 781-785 |
| ISSN: | 0975-1009 (Online); 0019-5189 (Print) |
| Appears in Collections: | IJEB Vol.39(08) [August 2001] |
Files in This Item:
| File | Description | Size | Format | |
|---|---|---|---|---|
| IJEB 39(8) 781-785.pdf | 1.17 MB | Adobe PDF | View/Open |
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