Please use this identifier to cite or link to this item:
http://nopr.niscpr.res.in/handle/123456789/23919| Title: | Detection of Theileria annulata carrier cattle by PCR |
| Authors: | Roy, K C Ray, D Bansal, G C Singh, R K |
| Issue Date: | Mar-2000 |
| Publisher: | NISCAIR-CSIR, India |
| Abstract: | A simple method for treating bovine blood samples for direct detection of T. annulata in carriers, after polymerase chain reaction (PCR) amplification of small subunit ribosomal RNA (SSU rRNA) gene is described. The threshold of detection of the PCR-assay was an erythrocytic parasitaemia of 0.00008% corresponding to 16 infected bovine erythrocytes. In 50 known carriers, 42 were positive in PCR, in which 8 cattle revealed presence of T. annulata in stained blood smear under microscope. |
| Page(s): | 283-284 |
| ISSN: | 0975-1009 (Online); 0019-5189 (Print) |
| Appears in Collections: | IJEB Vol.38(03) [March 2000] |
Files in This Item:
| File | Description | Size | Format | |
|---|---|---|---|---|
| IJEB 38(3) 283-284.pdf | 328.56 kB | Adobe PDF | View/Open |
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