Please use this identifier to cite or link to this item:
http://nopr.niscpr.res.in/handle/123456789/23982| Title: | Partial characterization of serum immunoglobulins of Oreochromis mossambicus |
| Authors: | Rajavarthini, P.Beulah Arunkumar, R. Isaac Michael, R. Dinakaran |
| Issue Date: | Jun-2000 |
| Publisher: | NISCAIR-CSIR, India |
| Abstract: | Serum immunoglobulins of O. mossambicus were purified using chromatography methods - CM affinity gel blue chromatography followed by two step purification involving a combination of ion-exchange and gel filtration chromatography. Studies revealed that O. mossambicus produces only one class of high molecular weight macroglobulin as determined by molecular sieving by Sepharose CL 6-B. Immunoelectrophoresis of purified O. mossambicus serum against rabbit anti O. mossambicus serum gave only a single precipitin line. Further analysis of the immunoglobulin by SDS-PAGE showed that the IgM macroglobulin weighs about 900,000 Da, composed of μ-like heavy chain weighing about 90 kDa each and light chains weighing about 30 kDa each. |
| Page(s): | 549-553 |
| ISSN: | 0975-1009 (Online); 0019-5189 (Print) |
| Appears in Collections: | IJEB Vol.38(06) [June 2000] |
Files in This Item:
| File | Description | Size | Format | |
|---|---|---|---|---|
| IJEB 38(6) 549-553.pdf | 1.04 MB | Adobe PDF | View/Open |
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