<span style="font-size: 21.5pt;mso-bidi-font-size:14.5pt;font-family:"Times New Roman","serif"; color:black">Interspecific hybridization in <i>Brassica juncea </i>and <i>Brassica toumefortii </i>through embryo rescue and their evaluation for biotic and abiotic stress tolerance </span>

Abstract

<span style="font-size: 15.5pt;mso-bidi-font-size:8.5pt;font-family:" times="" new="" roman","serif";="" color:black"="">Interspecific hybrids were obtained in an otherwise incompatible cross <i>Brassica juncea </i>x <i>Brassica tournefortii </i>through <i>in vitro </i>culture of hybrid embryos. The best response was observed from culture of embryos excised 20 days after pollination on MS medium supplemented with kinetin, α-naphthylacetic acid, gibberellic acid, glutamine and casein

<span style="font-size: 15.5pt;mso-bidi-font-size:8.5pt;font-family:" times="" new="" roman","serif";="" color:black"="">hydrolysate. One hybrid plant had many distinct or intermediate characters. It was tolerant to aphid attack, exhibited irregularities in meiotic events and was partially fertile. The <span style="font-size: 16.0pt;mso-bidi-font-size:9.0pt;font-family:" times="" new="" roman","serif";="" color:black"="">F₂ <span style="font-size:15.5pt;mso-bidi-font-size: 8.5pt;font-family:" times="" new="" roman","serif";color:black"="">open pollinated and BC₁<span style="font-size:13.0pt;mso-bidi-font-size:6.0pt;font-family:" arial","sans-serif";="" color:black"=""> <span style="font-size:15.5pt;mso-bidi-font-size:8.5pt; font-family:" times="" new="" roman","serif";color:black"="">progenies showed a large diversity in their morphological traits and some promising plants with less aphid infection, drought tolerance and high yield were selected.

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