<i><span style="font-size:21.5pt;mso-bidi-font-size:14.5pt;font-family:"Times New Roman","serif"; color:black">In vitro </span></i><span style="font-size:21.5pt;mso-bidi-font-size: 14.5pt;font-family:"Times New Roman","serif";color:black">high frequency regeneration of plantlets of <i>Vigna mungo </i>and their <i>ex vitro </i>growth </span>

Abstract

<span style="font-size: 15.5pt;mso-bidi-font-size:8.5pt;font-family:" times="" new="" roman","serif";="" color:black"="">Of the five explants of <i><span style="font-size:16.0pt; mso-bidi-font-size:9.0pt;font-family:" times="" new="" roman","serif";color:black"="">V. mungo </span></i><span style="font-size:15.5pt;mso-bidi-font-size:8.5pt; font-family:" times="" new="" roman","serif";color:black"="">var. T9 used, the excised shoot tips gave best response with regard to offshoot formation followed by the embryonal axis explants. While a treatment comprising 0.5 mgL^-1 BAP, 0.5 mgL^-12iP and 0.1 mgL^-1 NAA induced differentiation of an average 10 offshoots in shoot tip explants, only 3 offshoots were formed in the explants of embryonal axis in a treatment containing 0.5 mgL^-1 BAP and 0.1 mgL^-1 NAA, found optimum for them. Multiple shoots differentiated when explants with earlier regenerated and growing offshoots were first cultured in a treatment containing 0.1 mgL^-1 BAP, 0.25 mgL^-1 IAA and 5 mgL^-1 CCC and then subcultured in the same treatment but having only 1 mgL^-1 CCC. The isolated shoots rooted in 0.5 mgL^-1 IAA resulted in the formation of complete plantlets of an average height of 15 cm in 20 days. The <i><span style="font-size:16.0pt;mso-bidi-font-size:9.0pt;font-family:" times="" new="" roman","serif";="" color:black"="">in vitro, </span></i><span style="font-size:15.5pt;mso-bidi-font-size: 8.5pt;font-family:" times="" new="" roman","serif";color:black"="">regenerated plants grew normally under field conditions and came to flowering as well.

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