Please use this identifier to cite or link to this item:
http://nopr.niscpr.res.in/handle/123456789/24036| Title: | In vitro regeneration of Hypericum patulum Thunb.-A medicinal plant |
| Authors: | Baruah, Anjana Sarma, D Saud, J. Singh, R S |
| Issue Date: | Sep-2001 |
| Publisher: | NISCAIR-CSIR, India |
| Abstract: | A protocol was developed for high frequency plant regeneration in H. patulum by shoot-tip culture. H. patulum plants were collected from a wild source growing at high altitude in the eastern Himalayas. Multiple buds were initiated from shoot-tips cultured on Murashige and Skoog's medium supplemented with BAP, kinetin. Addition of thiamin HCI, Ca-pantothenate and biotin enhanced multiple shoot formation. Upon transfer to phytohormone free liquid medium following a brief exposure to auxin, root formation occurred from the micro shoots. Rooted plants were hardened and transferred to soil. Regeneration potentiality was found to be constant throughout the year in long term cultures. |
| Page(s): | 947-949 |
| ISSN: | 0975-1009 (Online); 0019-5189 (Print) |
| Appears in Collections: | IJEB Vol.39(09) [September 2001] |
Files in This Item:
| File | Description | Size | Format | |
|---|---|---|---|---|
| IJEB 39(9) 947-949.pdf | 643.51 kB | Adobe PDF | View/Open |
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