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http://nopr.niscpr.res.in/handle/123456789/24039| Title: | A dot-immunobinding assay (Dot-Iba) for rapid diagnosis of pulmonary tuberculosis |
| Authors: | Sumi, M G Mathai, A Reuben, S Radhakrishnan, V V Sasikumar, S Jayapal, V Felix, J |
| Issue Date: | Oct-2001 |
| Publisher: | NISCAIR-CSIR, India |
| Abstract: | IgG antibody to Mycobacterium tuberculosis from the sera of patients with 'definite' pulmonary tuberculosis (PT) was isolated and coupled with Cyanogen bromide-Sepharose 4B.Using an immunoabsorbent affinity chromatography, 14 kDa antigen was recovered from the culture filtrates of M. tuberculosis. With this mycobacterial antigen, a dot immunobinding assay (Dot-Iba) was developed for the detection of specific antibody to M. tuberculosis in the sera of patients with PT and controls. The assay gave positive results in all the 12 sputum-smear positive [acid fast bacilli (AFB)] patients with PT and gave negative results in the 50 sera from control groups. The Dot-Iba as described in this study, is simple, rapid and specific for laboratory diagnosis of PT. |
| Page(s): | 984-988 |
| ISSN: | 0975-1009 (Online); 0019-5189 (Print) |
| Appears in Collections: | IJEB Vol.39(10) [October 2001] |
Files in This Item:
| File | Description | Size | Format | |
|---|---|---|---|---|
| IJEB 39(10) 984-988.pdf | 1.23 MB | Adobe PDF | View/Open |
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