Characterization of toxin from cheilanthes fern and its effect on lymphocyte proliferation and DNA fragmentation

Abstract

<span style="font-size: 15.5pt;mso-bidi-font-size:8.5pt;font-family:" times="" new="" roman","serif""="">Thin layer chromatography of aqueous extract of whole <i><span style="font-size:15.0pt;mso-bidi-font-size:8.0pt;font-family:" times="" new="" roman","serif""="">Cheilanthes farinosa </span></i><span style="font-size:15.5pt;mso-bidi-font-size:8.5pt; font-family:" times="" new="" roman","serif""="">fern indicated the presence of ptaquiloside or ptaquiloside like compound, coinciding Rf values with that of Pterosin B standard. HPLC analysis revealed the presence of 26.3 mg/kg ptaquiloside. <i><span style="font-size:15.0pt;mso-bidi-font-size:8.0pt; font-family:" times="" new="" roman","serif""="">In vitro </span></i><span style="font-size:15.5pt;mso-bidi-font-size:8.5pt;font-family:" times="" new="" roman","serif""="">studies of the aqueous extract on lymphocyte culture revealed a correlation between stimulative indices and concentration of aqueous extract. Stimulation in lymphocyte proliferation was in order of bracken> cheilanthes > ConA> ptaquiloside standard. On incubation of lymphocyte with aqueous extract of ferns, no DNA damage was observed in isolated DNA.

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