RAPD markers linked to brown planthopper <i>Nilaparvatha lugens </i>resistance locus in rice

Abstract

Brown plant hopper, a major pest in rice causes ‘‘hopper burn’’ in the field. The resistance gene for brown planthopper was mapped by using 20 recombinant inbred lines (RIL's) derived from a cross between resistant line <i>Oryza. officinalis</i> derivative (IR 54742-2-21-12-17-6) and a susceptible rice cultivar ASD 16 using bulked segregant analysis. On an average

of 4 loci were amplified and two RAPD primers amplified loci that co-segregated with resistance/susceptibility. The segregating RAPD loci were mapped using Mapmaker programme into 13 groups. The expected and the 95% confidence level were found to be 15.2 and 47.7 eM respectively, confirming the location of the brown planthopper resistant gene on

the region of chromosome 4 . These RAPD markers will accelerate breeding programme for brown planthopper resistance.