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http://nopr.niscpr.res.in/handle/123456789/24131| Title: | L-Asparaginase activity in Aeromonas sp. isolated from freshwater mussel |
| Authors: | Pattnaik, Sophia Kabi, Rashmi Ram, K Janaki Bhanot, K K |
| Issue Date: | Nov-2000 |
| Publisher: | NISCAIR-CSIR, India |
| Abstract: | Aeromonas sp. from Lamellidens marginalis produced L-asparaginase when grown at 37°C. The optimum enzyme activity was at pH 9 when temperature was 45°C. Half-life of partially purified enzyme at 50°C and 55°C was 35 and 20 min, respectively. Activation and deactivation energies of partially purified enzyme were 17.48 and 24.86 kcal mol-1 respectively. The enzyme exhibited a Km(L-asparagine) value of 4.9 ×10-6 mol 1-1 and a Vmax of 9.803 IU ml-1. Three metal ions inhibited the enzyme activity at 10-20 μmol-1 concentrations. Catalytic activity was also inhibited by EDTA, iodoacetic acid, parach loromercuribenzoic acid and phenylmethylsulphonyl fluoride at 0.1 μ mol 1-1. |
| Page(s): | 1143-1146 |
| ISSN: | 0975-1009 (Online); 0019-5189 (Print) |
| Appears in Collections: | IJEB Vol.38(11) [November 2000] |
Files in This Item:
| File | Description | Size | Format | |
|---|---|---|---|---|
| IJEB 38(11) 1143-1146.pdf | 848.67 kB | Adobe PDF | View/Open |
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