<span style="font-size:12.0pt;font-family:"Times New Roman";mso-ansi-language:NL" lang="NL">Rapid micropropagation of <i>Geodorum densiflorum </i>(Lam) Schltr. in liquid culture </span>

Abstract

Technique for rapid mass propagation of <i>Geodorum densiflorum </i>(Lam)Schltr. has been developed by using thin sections of stems of <i>in vitro </i>regenerated plantlets as explant source. Thin sections of stems (1.0-1.5 mm) when cultured in modified liquid and semisolid Knudson C (KnC) medium produced 1.8 and 1.2 protocorm like bodies (PLBs) per explants respectively. Peptone (2 g 1^-1) was effective in promoting the survival percentage of

the explants but had no effect on PLB production. BAP and NAA when used individually enhanced the rate of PLB production. But a significant and manifold increase in PLB production was noted when BAP (3 mg 1^-1) and NAA (0.5 mg 1^-1) in combination were added to peptone supplemented liquid and semisolid KnC medium. PLBs thus obtained were subcultured in semisolid KnC medium and obtained well developed plantlets within 10-12

weeks.