Somatic embryogenesis in <i>Vigna radiata </i>(L.) Wilczek

Abstract

Somatic embryogenesis was achieved from immature cotyledon derived callus of mungbean, <i>V.radiata </i>(L.) Wilczek in MS liquid medium. Embryogenic callus was induced on MS medium with NAA (5mg/L).<i> </i>Differentiation of somatic embryos was observed when embryogenic callus was transferred to MS liquid medium containing 2, 4-D (1.5 mg/L) and L-proline (50 mg/L).<i> </i>The torpedo shaped embryos were transferred to MS liquid medium with BAP and ABA (1 mg/L<i> </i>each) for maturation and germination. Fifty per cent of torpedo shaped embryos were converted into tiny plants (8-9 plants out of 17) after one week of culture. The germinated embryos were isolated and transferred to MS half strength basal (solid) medium for further development.