<span style="font-size:15.0pt;mso-bidi-font-size:12.0pt" lang="EN-GB">Potential application of β-1, 3 glucanase from an environmental isolate of <i style="mso-bidi-font-style: normal">Pseudomonas aeruginosa</i> MCCB 123 in fungal DNA extraction </span>

Abstract

<i style="mso-bidi-font-style: normal"><span style="font-size:9.0pt;mso-bidi-font-size:12.0pt" lang="EN-GB">Pseudomonas aeruginosa</span></i><span style="font-size:9.0pt;mso-bidi-font-size: 12.0pt" lang="EN-GB"> MCCB 123 was grown in a synthetic medium for β-1,3 glucanase production. From the culture filtrate, β-1,3 glucanase was purified with a molecular mass of 45 kDa. The enzyme was a metallozyme as its β-1,3 glucanase activity got inhibited by the metal chelator EDTA. Optimum pH and temperature for β-1,3 glucanase activity on laminarin was found to be 7 and 50 °C respectively. The MCCB 123 β-1,3 glucanase was found to have good lytic action on a wide range of fungal isolates, and hence its application in fungal DNA extraction was evaluated. β-1,3 glucanase purified from the culture supernatant of <i style="mso-bidi-font-style: normal">P. aeruginosa</i> MCCB 123 could be used for the extraction of fungal DNA without the addition of any other reagents generally used. Optimum <i style="mso-bidi-font-style:normal">p</i>H and temperature of enzyme for fungal DNA extraction was found to be 7 and 65 °C respectively. This is the first report on β-1,3 glucanase employed in fungal DNA extraction<i style="mso-bidi-font-style: normal">.</i>

</span>