Restriction profile of amplified mitochondrial DNA of wild shrimp <span style="font-size:14.0pt;line-height:115%;font-family:"Times New Roman"; mso-fareast-font-family:"Times New Roman";color:black;mso-ansi-language:EN-IN; mso-fareast-language:EN-IN;mso-bidi-language:HI" lang="EN-IN">(<i>Penaeus monodon </i>Fabricius) populations of India</span>

Abstract

Mitochondrial DNA (mtDNA) ribosomal genes of <i>Penaeus monodon </i>collected from two wild populations of east coast and west coast of south India were amplified by polymerase chain reaction (PCR), which showed amplified DNA products of 415 base pairs (bp) size and 520 bp size for 12s ribosomal DNA (12s rDNA) and 16s ribosomal DNA (16s rDNA) genes respectively. Restriction endonuclease analysis of the amplified mtDNA ribosomal genes was used to assess the genetic variation among the two wild populations of <i>Penaeus monodon . </i>Alu I, Cla I and Hae III showed monomorphism suggesting

<span style="font-size:14.0pt;line-height:115%;font-family:" times="" new="" roman";="" mso-fareast-font-family:"times="" roman";color:black;mso-ansi-language:en-in;="" mso-fareast-language:en-in;mso-bidi-language:hi"="" lang="EN-IN">the existence of genetic variation between the two wild shrimp populations studied. Hence, the restriction enzyme analysis of mtDNA in identifying the shrimp populations may be used as a valuable technique for formulating shrimp breeding programmes.</span>