Application of Box-Benhken experimental design for optimization of laccase production by Coriolus versicolor MTCC138 in solid-state fermentation

Abstract

Ligninocellulosic waste and cyanobacterial bloom was used for production of laccase by Coriolus versicolor MTCC138 using Box- Benhken design method. Empirical model developed through RSM (Response surface methodology) in terms of effective operational factors of medium pH, temperature, moisture content, inducers, groundnut shell and cyanobacterial biomass was found adequate to describe production of laccase by C. versicolor MTCC138 in solid state fermentation. Correlation coefficient (0.9758) for laccase production indicated a good agreement between experimental and predicted values. Optimal physico-chemical factors for laccase production were found to be: cyanobacterial biomass, 2.55 g; pH, 4.94; temperature, 29.96 C; moisture content, 70.96 % and CuSO₄, 1.58 mM. Due to high potentiality of laccase, C. versicolor can be exploited industrially for developing a novel technology using inexpensive renewable resources. Box-Benhken approach appeared potential in process application.