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Title: Tinospora cordifolia (Willd.) Miers ex Hook.f. & Thoms.- plant tissue culture and comparative chemo-profiling study as a function of different supporting trees
Authors: Bhalerao, Bharat M
Vishwakarma, Kishor S
Maheshwari, Vijay L
Keywords: Tinospora cordifolia;Micropropagation;Secondary metabolite;Chromatography;Spectropscopy
Issue Date: Dec-2013
Publisher: NISCAIR-CSIR, India
IPC Code: Int. cl. (2011.01)−A61K 36/00
Abstract: Tinospora cordifolia (Willd.) Miers ex Hook. f. & Thoms., family Menispermaceae is an important medicinal plant, grows as a climber on various trees. In callus induction studies from leaf explants, the best results were obtained with 2, 4-D alone or in combination with kinetin. The calli thus obtained grew in size with time in culture medium but failed to differentiate. Shoot induction from nodal explants was best achieved on Murashige and Skoog basal medium with kinetin (8 μM) or kinetin and BAP in combination (12 and 2 μM, respectively). Rhizogenesis on regenerated shoots was induced by transferring them into medium fortified with NAA at 8 µM concentration. As per Ayurveda, the plant growing with physical support of Azadirachta indica A. Juss., (Neem) is medicinally more potent. In comparative chemical fingerprinting analyses of T. cordifolia growing with support of four different trees, viz. A. indica A. Juss., Butea monosperma (Lam.) Kuntze, Acacia leucophloea Willd., Prosopis juliflora DC, from the identical geo-climatic conditions, the ethanolic stem extracts showed identical band pattern/spectra in TLC, HPTLC, IR and UV spectroscopy. However, in IR and HPTLC analyses an additional minor peak/band at wave number 1271.08 and at Rf. 0. 97, respectively was seen in the extract of plant growing with the support of neem tree.
Page(s): 380-386
ISSN: 0976-0512 (Online); 0976-0504 (Print)
Appears in Collections:IJNPR Vol.4(4) [December 2013]

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