Combination of natural active substances and IL-2 to induce 293T cells to express the stem cell marker antigens

Abstract

<span style="font-size:11.0pt;font-family: " times="" new="" roman";mso-fareast-font-family:"times="" roman";mso-ansi-language:="" en-gb;mso-fareast-language:en-us;mso-bidi-language:hi"="" lang="EN-GB">Earlier, natural active substances (fish oocyte extract) were used to induce mouse fibroblast cells to express stem cell marker antigens. Further, in the present study, authors have tested the ability of these natural active substances in combination with IL-2 to induce 293T cells to express stem cell marker antigens. It was demonstrated that treatment with the natural substance in combination with IL-2 caused significant increase in the expression of Oct-3/4, c-Myc and Nanog marker antigens in 293T cells. The present approach was proved to be a rapid method to generate multipotent stem cells.</span>