Development of SCAR based molecular markers for identification of different species of <i style="mso-bidi-font-style:normal">Azolla</i>

Abstract

<i style="mso-bidi-font-style:normal">Azolla</i> is commonly found in rice paddy fields where it serves as a biofertilizer and green manure. The conventional methods of identification of <i style="mso-bidi-font-style:normal">Azolla</i> based on the presence or absence of glochidia on the spores is difficult and cumbersome. In the present study, therefore, molecular markers based on <span style="mso-fareast-font-family: Calibri" lang="EN-GB">specific sequence characterized amplified region (SCAR) primers were developed from the nucleotide sequence of specific RAPD markers. A single distinct and highly resolved band of 390, 182, 490 and 709 bp, respectively was obtained for <i style="mso-bidi-font-style:normal">Azolla rubra</i>,<i style="mso-bidi-font-style:normal"> A. pinnata</i>, <i style="mso-bidi-font-style:normal">A. filiculoides</i> and <i style="mso-bidi-font-style: normal">A. microphylla</i>. This confirms the specificity of SCAR primer for these species. Thus, the development of SCAR marker may aid in the accurate and precise identification of different species of <i style="mso-bidi-font-style: normal">Azolla</i> without ambiguity.

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