Molecular analysis for genetic structure of biotic and abiotic stress resistant genotypes in chickpea (<i style="mso-bidi-font-style: normal">Cicer arietinum</i> L.)

Abstract

Molecular characterization of biotic (<i style="mso-bidi-font-style:normal">Ascochyta </i>blight, <i style="mso-bidi-font-style:normal">Fusarium</i> wilt & dry root rot) and abiotic (drought & salinity) resistant genotypes of chickpea was carried out using a specific set of 20 polymorphic STMS (sequence tagged microsatellite site) markers. The number of alleles ranged from 1 to 3 alleles per locus. The PIC (polymorphism information content) value ranged from 0.0 to 0.656 with an average of 0.268, indicating the considerable efficiency of markers for studying the polymorphism level. The primer GA-33 showed maximum PIC value (0.656), while the ten primers had the 0.0 value. The dendogram derived from the analysis had six clusters. Many pairs showed the maximum similarity (0.983), whereas accessions ICC-1392 and ICC-2065 showed the minimum similarity (0.900). The conservation of genotypes ICC-2580, ICC-1392 and ICC-2065 was recommended for their utilization in Middle-east Asia region chickpea improvement programme.<b style="mso-bidi-font-weight: normal"></b>