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http://nopr.niscpr.res.in/handle/123456789/26623| Title: | Cholesterol Binding in Schistocerca gregaria and Insect Cell Lines |
| Authors: | Upadhyay, Ravi Kant Dhar, Ravi Agarwal, H C |
| Issue Date: | Oct-2000 |
| Publisher: | NISCAIR-CSIR, India |
| Abstract: | Cholesterol binding affinities in different tissue homogenates of Schistocerca gregaria (Forskal) and insect cell line are reported. In the first set of experiment , binding could be observed in haemolymph, ovary, testis, fat body, midgut, mucin and serum protiens of both sexes of Schistocerca gregoria. The binding assays were conducted in the presence of labelled and unlabelled cholesterol. The total number of binding sites (Rt ) are found to be 4.77×10-8 mole/L while extracted proteins from 1st and IInd peaks show 7.10×10-9 and 12.8× 10-9 mole/L. The binding sites in delipidated mucin have shown some higher binding (2.97 × 10-8 mole/L) than the non-lipidated mucin (1.38 ×10-8 mole/L). Insect cell lines Sf-9 and BmN cells have kd value of 1.494×10-8 and 4.08×10-8 mole/L in crude homogenate. |
| Page(s): | 845-851 |
| ISSN: | 0975-1084 (Online); 0022-4456 (Print) |
| Appears in Collections: | JSIR Vol.59(10) [October 2000] |
Files in This Item:
| File | Description | Size | Format | |
|---|---|---|---|---|
| JSIR 59(10) 845-851.pdf | 1.48 MB | Adobe PDF | View/Open |
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