<span style="font-size:12.0pt;font-family:"Times New Roman";color:black;mso-bidi-font-weight: bold" lang="EN-US">Isolation of Cholesterol Binding Proteins from Cultured <i><span style="font-size:12.0pt;font-family:"Times New Roman";color:black;mso-bidi-font-weight: bold" lang="EN-US">Spodoptera frugiparda </span></i><span style="font-size:12.0pt; font-family:"Times New Roman";color:black;mso-bidi-font-weight:bold" lang="EN-US">(Sf-9) Cells </span></span>

Abstract

Extraction of cholesterol binding proteins from cultured insect cells is described.

These proteins are found in buffer soluble cell homogenates. The determination of cholesterol binding sites at cell surface is described by two methods: by centrifugation and by gel filtration. From the first method 1.27 ± 0.15 ×10^-8 mole/L binding sites are determined and from the second, 4.48 ± 0.84 × 10^-8 mole/L binding sites arc reported . By centrifugation method, soluble portion of cell culture homogenate is separated. From this soluble portion, two low molecular weight proteins of 14 kD and 18 kD are isolated on a gel filtration column (Sepharose CI-6B-200).