Ameliorative effect of septilin, an ayurvedic preparation against γ-irradiation-induced oxidative stress and tissue injury in rats

Abstract

<span style="font-size:9.0pt;mso-bidi-font-size: 12.0pt;color:#1F1410" lang="EN-US">Ionizing radiation is known to induce <span style="font-size:9.0pt;mso-bidi-font-size:12.0pt;background:white" lang="EN-US">multiple organ dysfunctions directly related to an increase of cellular oxidative stress, due to overproduction of reactive oxygen species (ROS)<span style="font-size:9.0pt;mso-bidi-font-size:12.0pt" lang="EN-US">. This study was aimed to investigate the effect of septilin (an ayurvedic poly-herbal formulation containing the principal herbs, namely <i>Commiphora wightii, Trinospora cordifolia, Rubia cardifolia, Emblica officinalis, Saussurea lappa </i>and<i> Glycyrrhiza glabra</i>)<i> </i>against whole body γ-irradiation-induced oxidative damage in hepatic and brain tissues in rats. Administration of septilin for 5 days (100 mg/kg) prior to radiation resulted in a significant increase in both superoxide dismutase (SOD) activity and total glutathione (GSH) level in hepatic and brain tissues, while serum high-density lipoprotein-cholesterol (HDL) was reduced by γ-irradiation. Also, septilin resulted in a significant decrease in NO(x), nitric oxide and malondialdehyde (MDA) levels in hepatic and brain tissues and a significant decrease in serum triglycerides, low-density lipoprotein-cholesterol (LDL) and total cholesterol levels and serum alanine aminotransferase (ALT), aspartate aminotransferase (AST) levels and alkaline phosphatase (ALP), γ-glutamyl transferase (GGT) activities, as well as serum tumor necrosis factor-alpha (TNF-α), compared to irradiated group. In conclusion, data obtained from this study indicated that septilin exhibited potential antioxidant activity and showed radioprotective effect against γ-radiation by preventing oxidative stress and scavenging free radicals.

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