<span style="font-size:11.0pt;font-family: "Times New Roman";mso-fareast-font-family:"Times New Roman";mso-bidi-font-family: Mangal;mso-ansi-language:EN-GB;mso-fareast-language:EN-US;mso-bidi-language: HI" lang="EN-GB">Impact of plant growth regulators (PGRs) on callogenesis and artemisinin content in <i style="mso-bidi-font-style:normal">Artemisia annua</i> L. plants</span>

Abstract

Callus cultures were initiated from leaf explants of <i style="mso-bidi-font-style:normal">Artemisia annua</i> L. plants for artemisinin production using Murashige and Skoog (MS) medium supplemented with different concentrations and combinations of plant growth regulators (PGRs), <i style="mso-bidi-font-style:normal">viz</i>., BAP, NAA, 2,4-D and TDZ. The combination of all PGRs at different concentrations showed a better response in induction of undifferentiated friable calli in the light phase as compared to the dark phase. The calli of transgenic plants <span style="mso-fareast-font-family: Calibri" lang="EN-GB">over-expressing <i style="mso-bidi-font-style:normal">hmgr</i> gene yielded a biomass of 0.13 g dw/explant in the light in comparison to 0.06 g dw/explant in the dark, when grown on MS medium supplemented with BAP (1.0 mg L^-1) and NAA (2.0 mg L^-1). The non-transgenic (untransformed plant) calli, however, yielded a biomass of 0.10 g dw/explant in the light when grown on MS medium supplemented with the same concentrations of BAP and NAA, while yield was 0.06 g dw/explant in the dark in MS medium supplemented with BAP (1.0 mg L^-1) and 2, 4-D (1.0 mg L^-1). Further, the maximum artemisinin content (0.006% on dw basis) was recorded in both transgenic and non-transgenic calli that were grown on MS medium supplemented with BAP (1.0 mg L^-1) and NAA (2.0 mg L^-1) under light phase.

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