Evidence of cross reaction potential of recombinant leptospira LipL41 protein

Abstract

The present study was undertaken to compare the cross reactive potentiality of expressed recombinant protein (LipL41) with lipopolysaccharide (LPS) antigen of leptospira against different serovars of leptospira. Antigens from both recombinant LipL41 and the isolated LPS from <i>Leptospira</i><i style="mso-bidi-font-style:normal"> icterohaemorrhagiae</i> were coated in latex beads and used to test the human sera samples of different serovars of leptospira using field based latex agglutination test. Recombinant antigen showed strong cross reaction with different serovars of leptospira, whereas LPS antigen showed very mild cross reaction with few serovars. This clearly indicates that outer membrane protein cross reacted with heterologous serovars and can be used as effective diagnostic agent as well as candidate antigen for development of vaccine for leptospira.