Detection and analysis of genetic variations in species of <i style="mso-bidi-font-style: normal">Plumbago </i>L. using RAPD markers

Abstract

In the present study, random amplified polymorphic DNA (RAPD) markers were used to measure genetic diversity of 3 species of <i style="mso-bidi-font-style:normal">Plumbago</i>,<i style="mso-bidi-font-style:normal"> viz.</i>, <i style="mso-bidi-font-style: normal">P.</i> <i style="mso-bidi-font-style:normal">zeylanica</i> L., <i style="mso-bidi-font-style:normal">P.</i> <i style="mso-bidi-font-style:normal">rosea</i> L. and <i style="mso-bidi-font-style:normal">P.</i> <i style="mso-bidi-font-style: normal">auriculata</i> Lam., grown in India. Thirty nine decamer oligonucleotide primers were used to amplify the genomic DNA isolated from leaf samples. Of the primers used, only 19 primers gave clear reproducible bands in various ranges. A total of 299 amplified bands were scored, of which 107 were found to be polymorphic bands (PPB), <i style="mso-bidi-font-style:normal">i.e.</i>, 35.79%. The data obtained from the amplified bands were used in NTSYS PC Version 2.1 software and a cladogram was constructed showing the genetic distance between species. The results reveal that <i style="mso-bidi-font-style: normal">P. rosea</i> and <i style="mso-bidi-font-style:normal">P. zeylanica</i> were present in the same cluster, whereas<i style="mso-bidi-font-style:normal"> P. auriculata</i> showed divergence. Nei’s and Li’s similarity coefficients and GSI were also calculated.