Standardization of an Ayurvedic Formulation- <i>Kalyanavleha</i> and estimantion of Curcumin using HPTLC

Abstract

Standardization of a compound ayurvedic formulation is essential for establishing the authenticity, quality and efficacy of Ayurvedic medicines/finished herbal product. <i>Kalyanavleha</i> is an polyherbal ayurvedic formulation used in aphasia and hoarseness containing eight medicinal plants, viz. <i>Curcuma longa</i> L., <i>Cyclospermum leptophyllum</i> (Pers.) Sprague, <i>Zingiber officinale</i> Roscoe, <i>Cuminum cyminum</i> L., <i>Glycyrrhiza glabra</i> L., <i>Saussurea costus </i>(Falc.) Lipsch., <i>Acorus calamus</i> L., <i>Piper longum</i> L. To achieve the desired aim three batches of <i>Kalyanavleha</i> were prepared in the lab as per standard protocol of Ayurvedic Formulary and were investigated by microscopy, physicochemical parameters and high performance thin layer chromatography (HPTLC). Curcumin was evaluated qualitatively and quantitatively as the biomarker of <i>Kalyanavleha</i> and it was estimated 0.25-0.26 % in the formulation.