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http://nopr.niscpr.res.in/handle/123456789/29150| Title: | Induction of in vitro microrhizomes using silver nitrate in Zingiber officinale Rosc. var. Baishey and Nadia |
| Authors: | Singh, Thingbaijam Dikash Chakpram, Lalleima Devi, Huidrom Sunitibala |
| Keywords: | Benzylaminopurine;Microrhizome;Naphthaleneacetic acid;Plant growth regulators;Silver nitrate;Zingiber officinale |
| Issue Date: | Apr-2014 |
| Publisher: | NISCAIR-CSIR, India |
| Abstract: | An improved and high frequency in vitro microrhizome induction system has been developed in two varieties of Zingiber officinale Rosc., viz., ‘Baishey’ and ‘Nadia’. The influence of plant growth regulators, sucrose concentration, type of culture vessel and presence or absence of silver nitrate on microrhizome production was investigated. Murashige and Skoog’s (MS) medium supplemented with 2 mg L-¹ 6-benzylaminopurine, 1 mg L-¹ α-naphthaleneacetic acid and 8 g L-¹ sucrose with the presence of 11 µM silver nitrate was found suitable for high frequency in vitro microrhizome production. Silver nitrate enriched culture medium showed remarkable increase in the number of in vitro microrhizome production per vessel (Baishey, 28.20±0.08 & Nadia, 27.25±0.10). The present study emphasized the role of silver nitrate and different culture vessels in the development of in vitro microrhizome within short period of time. Among the different culture vessels examined, growtek showed the highest average number of microrhizome (Baishey, 34.25±.09 & Nadia, 38.25±.09) production in both the varieties of ginger within 35 to 45 d of incubation. About 90% of Baishey and 93% of Nadia plantlets with silver nitrate and 75-85% plantlets without silver nitrate treatment in both the varieties survived during acclimatization under ex vitro conditions. |
| Page(s): | 256-262 |
| ISSN: | 0975-0967 (Online); 0972-5849 (Print) |
| Appears in Collections: | IJBT Vol.13(2) [April 2014] |
Files in This Item:
| File | Description | Size | Format | |
|---|---|---|---|---|
| IJBT 13(2) 256-262.pdf | 503.17 kB | Adobe PDF | View/Open |
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