<span style="font-size:11.0pt;font-family: "Times New Roman";mso-fareast-font-family:"Times New Roman";mso-bidi-font-family: Mangal;mso-ansi-language:EN-GB;mso-fareast-language:EN-US;mso-bidi-language: HI" lang="EN-GB">Direct and callus mediated regeneration from nodal and internodal segments of <i style="mso-bidi-font-style:normal">Crataeva religiosa</i> G. Forst. var. <i>nurvala</i> (Buch.-Ham.) Hook. f. & Thomson</span>

Abstract

<i style="mso-bidi-font-style:normal">Crataeva religiosa</i> G. Forst. var. <i>nurvala</i> (Buch.-Ham.) Hook. F. & Thomson (syn<i style="mso-bidi-font-style:normal">. Crataeva</i> <i style="mso-bidi-font-style:normal">nurvala </i>Buch.-Ham.), a fast growing medicinal tree species, has been successfully micropropagated under <i style="mso-bidi-font-style:normal">in vitro</i> conditions. Direct multiple shoots were regenerated from nodal explants on Murashige and Skoog’s (MS) medium, solidified by agar and supplemented with 1 mg/L 6-benzyl amino purine (BAP) and 0.5 mg/L α-naphthalene acetic acid (NAA). However, the successful regeneration from internodal callus was achieved on 2.0 mg/L BAP and 0.5 mg/L NAA. The frequency of <i style="mso-bidi-font-style:normal">in vitro </i>multiplication from culture of nodal segments was higher compared to that of callus mediated regeneration from culture of internodal segments. Further, the best rhizogenesis was achieved on solidified MS medium supplemented with 3 mg/L indole-3-butyric acid (IBA).