<span style="font-size:13.0pt;mso-bidi-font-size: 8.0pt;letter-spacing:-.2pt" lang="EN-GB">Genetic fidelity assessment of micropropagated <i style="mso-bidi-font-style:normal">Spilanthes acmella</i> (L.) Murr. by RAPD and ISSR markers assay </span>

Abstract

Random amplified polymorphic DNA (RAPD) and inter-simple sequence repeats (ISSR) markers were employed to assess the genetic stability of <i style="mso-bidi-font-style:normal">Spilanthes acmella</i> (L.) Murr., plants multiplied through axillary bud multiplication with up to 20 <i style="mso-bidi-font-style:normal">in vitro</i> subcultures. The micropropagated plants were hardened with treatment of arbuscular mycorrhizal fungi (AMF), namely, <i style="mso-bidi-font-style:normal">Glomus mosseae</i><span style="mso-bidi-font-style:italic">,<i style="mso-bidi-font-style:normal"> </i>as biohardening agents to improve their survival and growth. During the study, a total of 50 (30 RAPD & 20 ISSR) primers were screened. Of which 19 RAPD and 12 ISSR primers produced a total of 129 (81 RAPD & 48 ISSR) clear, distinct and reproducible amplicons. The amplification products were monomorphic across all the selected micropropagated plants and were similar to the mother plant. These results indicate that the micropropagation protocol developed for rapid <i style="mso-bidi-font-style: normal">in vitro</i> multiplication of <i style="mso-bidi-font-style:normal">S. acmella</i> is appropriate for its clonal propagation. The outcome supports the fact that axillary bud multiplication can also be used as one of the safest modes for the production of true-to-type plants.

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