Antiangiogenic and antiproliferative assessment of cyanobacteria

Abstract

Biologically active compounds with different modes of action, such as antiproliferative, antioxidant, antimicrotubule, have been isolated from algae and cyanobacteria. The present study was designed to evaluate antiangiogenic and antiproliferative potential of dichloromethane and methanol (2:1) extracts of different cyanobacteria. Further fingerprinting of the activity possessing extracts were carried out using ESI-LC-MS/MS. Extracts (25, 50 and 100 µg) were screened in the vascular endothelial growth factor (VEGF) induced angiogenesis in <i style="mso-bidi-font-style:normal">inovo</i> chick chorioallontoic membrane assay (CAM) at various concentrations using thalidomide and normal saline as positive and untreated control groups respectively. The extracts were also evaluated for their antiproliferative activity by MTT assay using HeLa cancer cell line. The results obtained from the various algal extracts did not show any significant antiangiogenic activity as compared to VEGF control. <i><span style="letter-spacing:-.1pt;mso-ansi-language:EN-IN">Oscillatoria sp. </span></i><span style="letter-spacing:-.1pt;mso-ansi-language:EN-IN;mso-bidi-font-style:italic">and<i> </i><i>Lyngbya officinalis</i> exhibited significant anti-proliferative<span style="mso-bidi-font-style:italic"> activity at IC₅₀ values of <span style="mso-bidi-font-weight:bold">220 and 260 µg/mL respectively. ESI-LC-MS/MS of<i> L. officinalis</i><i style="mso-bidi-font-style:normal"><span style="mso-bidi-font-weight:bold"></span></i><span style="mso-bidi-font-weight: bold"> showed the presence of lyngbyatoxin-A and<span style="letter-spacing:-.1pt;mso-ansi-language:EN-IN;mso-bidi-font-style:italic"> that of <i>Oscillatoria sp.</i> reveals<span style="letter-spacing:-.1pt;mso-bidi-font-weight:bold" lang="EN-GB"> the presence of malyngamide-J suggesting the possibility of antiproliferative activity.

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