Effect of Nutritional Supplementation of Solid State Fermentation Medium on Biosynthesis of Phytase from <i style="mso-bidi-font-style:normal">Aspergillus niger</i> NCIM 612

Abstract

Extracellular phytase production by <i style="mso-bidi-font-style:normal">Aspergillus niger</i> NCIM 612 was studied under solid state fermentation (SSF). Effect of supplementation of nutrients, such as, carbon, nitrogen, inorganic phosphate, surfactant and metal salt, on phytase production was investigated. Results showed that glucose (2.5 % w/w) as carbon source, ammonium nitrate (0.5 % w/w) as nitrogen source, potassium dihydrogen phosphate (0.2 % w/w) as phosphate source and ferrous sulphate (0.01 % w/w) as metal source were found to enhance phytase production by 1.13 fold, 1.16 fold, 1.07 fold and 1.17 fold, respectively. None of the surfactants favored phytase yield.