<span style="font-size:11.0pt;font-family:"Calibri","sans-serif"; mso-ascii-theme-font:minor-latin;mso-fareast-font-family:"Times New Roman"; mso-fareast-theme-font:minor-fareast;mso-hansi-theme-font:minor-latin; mso-bidi-font-family:"Times New Roman";mso-ansi-language:EN-US;mso-fareast-language: EN-US;mso-bidi-language:AR-SA">Increased metal tolerance by <i>Thiobacillus ferrooxidans </i>(SRco) isolated from Ambamata complex ore mine</span>

Abstract

Two isolates of <i>T. ferrooxidans </i>(SRco) cultures 1

and 2 have been developed to tolerate Cd²⁺, Co²⁺,

Cu²⁺ and Ni²⁺ in different conditions. Cadmium, in

the form of cadmium sulphate, has been found to be

most tolerated by the organisms, followed by copper,

nickel and cobalt in the form of sulphate salts. Ni²⁺

and Co²⁺ are tolerated equally by the organisms, but

organisms needed more time to oxidize 95%

Fe²⁺- Fe³⁺ in presence of Co²⁺ as compared to Ni²⁺. Cu²⁺ is found to be most inhibitory even at low concentrations while Co²⁺ shows inhibitory action at

higher concentration. Isolates have been developed to tolerate as high as (and show 95% iron oxidation)

1.75, 1.527, 1.254 and 1.254% (correspond to 16, 24,

<span style="font-size:10.0pt;font-family:" times="" new="" roman","serif";mso-fareast-font-family:="" "times="" roman";mso-fareast-theme-font:minor-fareast;mso-ansi-language:en-us;="" mso-fareast-language:en-us;mso-bidi-language:ar-sa"="">21, 21 m<i style="mso-bidi-font-style:normal">M</i>) cadmium, copper, nickel and cobalt, respectively.</span>