Energy restriction and impact on indirect calorimetry and oxidative stress in cardiac tissue in rat

Abstract

Caloric restriction, defined as a reduction in calorie intake below <i style="mso-bidi-font-style:normal">ad libitum</i>, without malnutrition can have beneficial effects. In this study, we evaluated the impact of caloric restriction of 30 and 60% on calorimetric parameters and oxidative stress in cardiac tissue in rats. Rats were randomly divided into 3 groups (<i>n</i> = 8): G1 = control; G2 = rats exposed to dietary restriction of 30%; and G3 = rats exposed to dietary restriction of 60%. <span style="font-size:9.0pt;mso-ansi-language:EN-GB" lang="EN-GB">Energy restriction decreased final body weight, oxidation of carbohydrates and lipid, oxygen consumption (VO₂), carbon dioxide production (VCO₂), resting metabolic rate (RMR), but elevated respiratory quotient (RQ). G3 animals also displayed an imbalance in the oxidant/antioxidant system, as revealed by the decrease in the lipid hydroperoxide (LH) level and GSH-Px activity in heart tissue. In conclusion, dietary restriction decreased oxidative metabolism, as seen by the colorimetric profiles and controlled oxidative<span style="font-size:9.0pt;mso-ansi-language:EN-GB" lang="EN-GB"> stress in cardiac tissue.

</span></span>