Analysis on the arcelin expression in bruchid pest resistant wild pulses using real time RT-qPCR

Abstract

<span style="font-size:9.0pt;mso-bidi-font-size:12.0pt" lang="EN-GB">Arcelin, the antimetabolic protein from wild pulses is a known natural insecticidal molecule. Wild pulses with high arcelin content could serve as potential source to increase the levels of insect resistance in cultivated pulse crops. In this study, arcelin (<i style="mso-bidi-font-style:normal">Arl</i>) gene expression was screened in seven stored product insect pest resistant wild pulse varieties using real time RT-qPCR. Arcelin gene specific real time PCR primers were synthesized from arcelin mRNA sequence of the wild pulse variety, <i style="mso-bidi-font-style: normal">Lablab</i> <i style="mso-bidi-font-style:normal">purpureus</i>. The results revealed different levels of arcelin gene expression in the tested varieties. <i style="mso-bidi-font-style:normal"><span style="font-size:9.0pt;mso-bidi-font-size:12.0pt;mso-bidi-language:TA" lang="EN-GB">Canavalia virosa</span></i><span style="font-size:9.0pt;mso-bidi-font-size: 12.0pt" lang="EN-GB"> registered significantly high content indicating its suitability for utilization of arcelin gene in developing stored product insect pest resistance with other cultivated pulses.

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