Anti-bacterial properties of lactoferrin immobilized wool fabric

Abstract

A new biological cross-linker, microbial transglutaminase <span style="mso-fareast-font-family:" ms="" mincho";mso-bidi-font-family:"ms="" mincho""="" lang="EN-GB">(mTGase), <span style="mso-bidi-font-family:" times="" new="" roman""="" lang="EN-GB">has been used to catalyze the<span style="mso-bidi-font-weight:bold"> immobilization of lactoferrin onto the wool fabrics, and the antibacterial properties of immobilized wool on both Gram-negative and Gram-positive bacteria are studied. It is found that the minimal inhibitory concentration of lactoferrin against <i style="mso-bidi-font-style: normal">S·aureus</i> and <i style="mso-bidi-font-style: normal">E·coli</i> is 0.5mg/mL and 0.25mg/mL respectively. As compared to the control sample, the amount of lactoferrin adhered onto the wool fabric improves from 4.87 mg^{<span style="mso-fareast-font-family:SimHei" lang="EN-GB">.</span>}(g fabric)^-1 to 12.96 mg^{<span style="mso-fareast-font-family: SimHei" lang="EN-GB">.</span>}(g fabric)^-1, indicating that the crosslinking reaction initiated by mTGase can increase the amount of lactoferrin fixed onto wool fabric obviously. The ratios of bacteriostasis to <i style="mso-bidi-font-style:normal">S.aureus</i> and <i style="mso-bidi-font-style: normal">E.coli</i> of wool fabrics immobilized with lactoferrin are bound to be 57.95% and 69.96% respectively, showing good antibacterial property.

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