<span style="mso-bidi-language:HI">Temporal expression patterns of <i>timeless </i>in <i>vg </i>and <i>cry^b </i>mutants of <i>Drosophila melanogaster</i> </span>

Abstract

<span style="mso-bidi-language:HI">The temporal expression patterns of <i>timeless (tim) </i>in <i>Drosophila melanogaster </i>at various time points were studied in intestine and salivary gland of wild type (WT), <i>vestigial (vg) </i>and <i>cryptochrome-absent </i>(<i>cry^b</i>)<i> </i>mutants under 12 hr:12 hr

<span style="mso-bidi-language:HI">white light:darkness (LD) and 12 hr:12 hr blue light (450 nm):darkness (BD) conditions. At ZT 06 and ZT 10, <i>tim </i>expression was almost nil and at ZT 18 and ZT 22, the expression was most pronounced in WT and mutants, when compared to other time points. As <i>vg </i>flies have greatly reduced wings, their gross locomotor activity was poorer and levels of <i>tim </i>expression were also least than WT flies. The weaker expression of <i>tim </i>in <i>cry^b </i>flies suggested the significant role of blue light photoreceptor <i>cryptochrome </i>for a stronger synchronization of circadian clock. The expression patterns of <i>rim </i>in the salivary gland of larvae further suggested the presence of peripheral oscillators during the developmental stages.

</span></span>